Environmental Sequencing Facility

Sequencing capabilities

• Our in-house sequencing platforms, the Illumina MiSeq i100 Plus and Oxford Nanopore MinION, provide versatile capabilities for various research applications.
• The Miseq i100 Plus supports flexible read lengths (2x100bp – 2x500bp) and can generate between 5 million to 100 million ultra-high quality read-pairs per run, accommodating a wide range of project parameters.
• Our portable MinION devices can generate reads spanning a few hundred to hundreds of thousands of bases in length and allow for both amplification-based sequencing and PCR-free sequencing of native nucleic acids.

These sequencing platforms are suitable for:
 

• Amplicon sequencing and metabarcoding
• Environmental DNA (eDNA) tracking and surveillance
• Whole genome sequencing of small genomes
• RNA sequencing
• Methylation profiling
• Smaller scale shotgun metagenomics and metatranscriptomics

We also offer standalone library preparation or can offer external sequencing options for alternative high throughput sequencing needs. Please contact us to discuss the best sequencing options and specific nucleic acid sample requirements for your project.

Other key instruments include:
 

• Agilent 4200 Tapestation for automated nucleic acid quality control
• Biomek 4000 liquid handling robot and Formulatrix Mantis for automated liquid handling
• Roche LightCycler qPCR instruments for reliable qPCR and RT-qPCR testing

Bioinformatics services

• Sequence data quality control and pre-processing
• Community metabarcoding analysis
• Metagenomics
• Metatranscriptomics
• Whole genome sequencing pipelines

Through the use of our own dedicated high performance computing cluster, we can support your bioinformatics needs for projects of all sizes and scopes.